Endothelial mechanotransduction proteins and vascular function are altered by dietary sucrose supplementation in healthy young male subjects

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Endothelial mechanotransduction proteins and vascular function are altered by dietary sucrose supplementation in healthy young male subjects. / Gliemann, Lasse; Rytter, Nicolai; Lindskrog, Mads; Slingsby, Martina Helena Lundberg; Åkerström, Thorbjörn; Sylow, Lykke; Richter, Erik A.; Hellsten, Ylva.

I: Journal of Physiology, Bind 595, Nr. 16, 2017, s. 5557-5571.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Gliemann, L, Rytter, N, Lindskrog, M, Slingsby, MHL, Åkerström, T, Sylow, L, Richter, EA & Hellsten, Y 2017, 'Endothelial mechanotransduction proteins and vascular function are altered by dietary sucrose supplementation in healthy young male subjects', Journal of Physiology, bind 595, nr. 16, s. 5557-5571. https://doi.org/10.1113/JP274623

APA

Gliemann, L., Rytter, N., Lindskrog, M., Slingsby, M. H. L., Åkerström, T., Sylow, L., Richter, E. A., & Hellsten, Y. (2017). Endothelial mechanotransduction proteins and vascular function are altered by dietary sucrose supplementation in healthy young male subjects. Journal of Physiology, 595(16), 5557-5571. https://doi.org/10.1113/JP274623

Vancouver

Gliemann L, Rytter N, Lindskrog M, Slingsby MHL, Åkerström T, Sylow L o.a. Endothelial mechanotransduction proteins and vascular function are altered by dietary sucrose supplementation in healthy young male subjects. Journal of Physiology. 2017;595(16):5557-5571. https://doi.org/10.1113/JP274623

Author

Gliemann, Lasse ; Rytter, Nicolai ; Lindskrog, Mads ; Slingsby, Martina Helena Lundberg ; Åkerström, Thorbjörn ; Sylow, Lykke ; Richter, Erik A. ; Hellsten, Ylva. / Endothelial mechanotransduction proteins and vascular function are altered by dietary sucrose supplementation in healthy young male subjects. I: Journal of Physiology. 2017 ; Bind 595, Nr. 16. s. 5557-5571.

Bibtex

@article{94a3ac8cdf394a1bbda34e8d901ad098,
title = "Endothelial mechanotransduction proteins and vascular function are altered by dietary sucrose supplementation in healthy young male subjects",
abstract = "Endothelial mechanotransduction is important for vascular function but alterations and activation of vascular mechanosensory proteins have not been investigated in humans. In endothelial cell culture, simple sugars effectively impair mechanosensor proteins. To study mechanosensor- and vascular function in humans, twelve young healthy male subjects supplemented their diet with 3 × 75 g sucrose day(-1) for 14 days in a randomized cross-over design. Before and after the intervention period, the hyperemic response to passive lower leg movement and active knee extensor exercise was determined by ultrasound doppler. A muscle biopsy was obtained from the thigh muscle before and after acute passive leg movement, to asses the protein amount and phosphorylation status of mechanosensory proteins and NADPH oxidase. The sucrose intervention led to a reduced flow response to passive movement (by 17 ± 2 %) and to 12 watts of active exercise (by 9 ± 1 %), indicating impaired vascular function. Reduced flow response to passive and active exercise was paralleled by a significant upregulation of Platelet endothelial cell adhesion molecule (PECAM-1), endothelial nitric oxide synthase, NADPH oxidase and the Rho family GTPase Rac1 protein expression in the muscle tissue as well as an increased basal phosphorylation status of Vascular endothelial growth factor receptor 2 and a reduced phosphorylation status of PECAM-1. The phosphorylation status was not acutely altered with passive leg movement. These findings indicate that regular intake of high levels of sucrose can impair vascular mechanotransduction, increase the oxidative stress potential and suggest that dietary excessive sugar intake may contribute to the development of vascular disease. This article is protected by copyright. All rights reserved.",
keywords = "Vascular function, Passive leg movement, Mechanosensor",
author = "Lasse Gliemann and Nicolai Rytter and Mads Lindskrog and Slingsby, {Martina Helena Lundberg} and Thorbj{\"o}rn {\AA}kerstr{\"o}m and Lykke Sylow and Richter, {Erik A.} and Ylva Hellsten",
note = "CURIS 2017 NEXS 222",
year = "2017",
doi = "10.1113/JP274623",
language = "English",
volume = "595",
pages = "5557--5571",
journal = "The Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "16",

}

RIS

TY - JOUR

T1 - Endothelial mechanotransduction proteins and vascular function are altered by dietary sucrose supplementation in healthy young male subjects

AU - Gliemann, Lasse

AU - Rytter, Nicolai

AU - Lindskrog, Mads

AU - Slingsby, Martina Helena Lundberg

AU - Åkerström, Thorbjörn

AU - Sylow, Lykke

AU - Richter, Erik A.

AU - Hellsten, Ylva

N1 - CURIS 2017 NEXS 222

PY - 2017

Y1 - 2017

N2 - Endothelial mechanotransduction is important for vascular function but alterations and activation of vascular mechanosensory proteins have not been investigated in humans. In endothelial cell culture, simple sugars effectively impair mechanosensor proteins. To study mechanosensor- and vascular function in humans, twelve young healthy male subjects supplemented their diet with 3 × 75 g sucrose day(-1) for 14 days in a randomized cross-over design. Before and after the intervention period, the hyperemic response to passive lower leg movement and active knee extensor exercise was determined by ultrasound doppler. A muscle biopsy was obtained from the thigh muscle before and after acute passive leg movement, to asses the protein amount and phosphorylation status of mechanosensory proteins and NADPH oxidase. The sucrose intervention led to a reduced flow response to passive movement (by 17 ± 2 %) and to 12 watts of active exercise (by 9 ± 1 %), indicating impaired vascular function. Reduced flow response to passive and active exercise was paralleled by a significant upregulation of Platelet endothelial cell adhesion molecule (PECAM-1), endothelial nitric oxide synthase, NADPH oxidase and the Rho family GTPase Rac1 protein expression in the muscle tissue as well as an increased basal phosphorylation status of Vascular endothelial growth factor receptor 2 and a reduced phosphorylation status of PECAM-1. The phosphorylation status was not acutely altered with passive leg movement. These findings indicate that regular intake of high levels of sucrose can impair vascular mechanotransduction, increase the oxidative stress potential and suggest that dietary excessive sugar intake may contribute to the development of vascular disease. This article is protected by copyright. All rights reserved.

AB - Endothelial mechanotransduction is important for vascular function but alterations and activation of vascular mechanosensory proteins have not been investigated in humans. In endothelial cell culture, simple sugars effectively impair mechanosensor proteins. To study mechanosensor- and vascular function in humans, twelve young healthy male subjects supplemented their diet with 3 × 75 g sucrose day(-1) for 14 days in a randomized cross-over design. Before and after the intervention period, the hyperemic response to passive lower leg movement and active knee extensor exercise was determined by ultrasound doppler. A muscle biopsy was obtained from the thigh muscle before and after acute passive leg movement, to asses the protein amount and phosphorylation status of mechanosensory proteins and NADPH oxidase. The sucrose intervention led to a reduced flow response to passive movement (by 17 ± 2 %) and to 12 watts of active exercise (by 9 ± 1 %), indicating impaired vascular function. Reduced flow response to passive and active exercise was paralleled by a significant upregulation of Platelet endothelial cell adhesion molecule (PECAM-1), endothelial nitric oxide synthase, NADPH oxidase and the Rho family GTPase Rac1 protein expression in the muscle tissue as well as an increased basal phosphorylation status of Vascular endothelial growth factor receptor 2 and a reduced phosphorylation status of PECAM-1. The phosphorylation status was not acutely altered with passive leg movement. These findings indicate that regular intake of high levels of sucrose can impair vascular mechanotransduction, increase the oxidative stress potential and suggest that dietary excessive sugar intake may contribute to the development of vascular disease. This article is protected by copyright. All rights reserved.

KW - Vascular function

KW - Passive leg movement

KW - Mechanosensor

U2 - 10.1113/JP274623

DO - 10.1113/JP274623

M3 - Journal article

C2 - 28620941

VL - 595

SP - 5557

EP - 5571

JO - The Journal of Physiology

JF - The Journal of Physiology

SN - 0022-3751

IS - 16

ER -

ID: 179559744