Development of an enrichment method for endogenous phosphopeptide characterization in human serum

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Development of an enrichment method for endogenous phosphopeptide characterization in human serum. / La Barbera, Giorgia; Capriotti, Anna Laura; Cavaliere, Chiara; Ferraris, Francesca; Laus, Michele; Piovesana, Susy; Sparnacci, Katia; Laganà, Aldo.

I: Analytical and Bioanalytical Chemistry, Bind 410, Nr. 3, 01.01.2018, s. 1177-1185.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

La Barbera, G, Capriotti, AL, Cavaliere, C, Ferraris, F, Laus, M, Piovesana, S, Sparnacci, K & Laganà, A 2018, 'Development of an enrichment method for endogenous phosphopeptide characterization in human serum', Analytical and Bioanalytical Chemistry, bind 410, nr. 3, s. 1177-1185. https://doi.org/10.1007/s00216-017-0822-8

APA

La Barbera, G., Capriotti, A. L., Cavaliere, C., Ferraris, F., Laus, M., Piovesana, S., ... Laganà, A. (2018). Development of an enrichment method for endogenous phosphopeptide characterization in human serum. Analytical and Bioanalytical Chemistry, 410(3), 1177-1185. https://doi.org/10.1007/s00216-017-0822-8

Vancouver

La Barbera G, Capriotti AL, Cavaliere C, Ferraris F, Laus M, Piovesana S o.a. Development of an enrichment method for endogenous phosphopeptide characterization in human serum. Analytical and Bioanalytical Chemistry. 2018 jan 1;410(3):1177-1185. https://doi.org/10.1007/s00216-017-0822-8

Author

La Barbera, Giorgia ; Capriotti, Anna Laura ; Cavaliere, Chiara ; Ferraris, Francesca ; Laus, Michele ; Piovesana, Susy ; Sparnacci, Katia ; Laganà, Aldo. / Development of an enrichment method for endogenous phosphopeptide characterization in human serum. I: Analytical and Bioanalytical Chemistry. 2018 ; Bind 410, Nr. 3. s. 1177-1185.

Bibtex

@article{ded9af6125624ba1980b9fbb36a089e0,
title = "Development of an enrichment method for endogenous phosphopeptide characterization in human serum",
abstract = "The work describes the development of an enrichment method for the analysis of endogenous phosphopeptides in serum. Endogenous peptides can play significant biological roles, and some of them could be exploited as future biomarkers. In this context, blood is one of the most useful biofluids for screening, but a systematic investigation of the endogenous peptides, especially phosphorylated ones, is still lacking, mainly due to the lack of suitable analytical methods. Thus, in this paper, different phosphopeptide enrichment strategies were pursued, based either on metal oxide affinity chromatography (MOAC, in the form of commercial TiO2 spin columns or magnetic graphitized carbon black-TiO2 composite), or on immobilized metal ion affinity chromatography (IMAC, in the form of Ti4+-IMAC magnetic material or commercial Fe3+-IMAC spin columns). While MOAC strategies proved completely unsuccessful, probably due to interfering phospholipids displacing phosphopeptides, the IMAC materials performed very well. Different sample preparation strategies were tested, comprising direct dilution with the loading buffer, organic solvent precipitation, and lipid removal from the matrix, as well as the addition of phosphatase inhibitors during sample handling for maximized endogenous phosphopeptide enrichment. All data were acquired by a shotgun peptidomics approach, in which peptide samples were separated by reversed-phase nanoHPLC hyphenated with high-resolution tandem mass spectrometry. The devised method allowed the identification of 176 endogenous phosphopeptides in fresh serum added with inhibitors by the direct dilution protocol and the Ti4+-IMAC magnetic material enrichment, but good results could also be obtained from the commercial Fe3+-IMAC spin column adapted to the batch enrichment protocol.",
keywords = "Endogenous serum phosphopeptides, Magnetic solid-phase extraction, NanoHPLC-MS/MS, Phosphopeptide enrichment, Phosphoproteomics, Ti-IMAC",
author = "{La Barbera}, Giorgia and Capriotti, {Anna Laura} and Chiara Cavaliere and Francesca Ferraris and Michele Laus and Susy Piovesana and Katia Sparnacci and Aldo Lagan{\`a}",
year = "2018",
month = "1",
day = "1",
doi = "10.1007/s00216-017-0822-8",
language = "English",
volume = "410",
pages = "1177--1185",
journal = "Analytical and Bioanalytical Chemistry",
issn = "1618-2642",
publisher = "Springer",
number = "3",

}

RIS

TY - JOUR

T1 - Development of an enrichment method for endogenous phosphopeptide characterization in human serum

AU - La Barbera, Giorgia

AU - Capriotti, Anna Laura

AU - Cavaliere, Chiara

AU - Ferraris, Francesca

AU - Laus, Michele

AU - Piovesana, Susy

AU - Sparnacci, Katia

AU - Laganà, Aldo

PY - 2018/1/1

Y1 - 2018/1/1

N2 - The work describes the development of an enrichment method for the analysis of endogenous phosphopeptides in serum. Endogenous peptides can play significant biological roles, and some of them could be exploited as future biomarkers. In this context, blood is one of the most useful biofluids for screening, but a systematic investigation of the endogenous peptides, especially phosphorylated ones, is still lacking, mainly due to the lack of suitable analytical methods. Thus, in this paper, different phosphopeptide enrichment strategies were pursued, based either on metal oxide affinity chromatography (MOAC, in the form of commercial TiO2 spin columns or magnetic graphitized carbon black-TiO2 composite), or on immobilized metal ion affinity chromatography (IMAC, in the form of Ti4+-IMAC magnetic material or commercial Fe3+-IMAC spin columns). While MOAC strategies proved completely unsuccessful, probably due to interfering phospholipids displacing phosphopeptides, the IMAC materials performed very well. Different sample preparation strategies were tested, comprising direct dilution with the loading buffer, organic solvent precipitation, and lipid removal from the matrix, as well as the addition of phosphatase inhibitors during sample handling for maximized endogenous phosphopeptide enrichment. All data were acquired by a shotgun peptidomics approach, in which peptide samples were separated by reversed-phase nanoHPLC hyphenated with high-resolution tandem mass spectrometry. The devised method allowed the identification of 176 endogenous phosphopeptides in fresh serum added with inhibitors by the direct dilution protocol and the Ti4+-IMAC magnetic material enrichment, but good results could also be obtained from the commercial Fe3+-IMAC spin column adapted to the batch enrichment protocol.

AB - The work describes the development of an enrichment method for the analysis of endogenous phosphopeptides in serum. Endogenous peptides can play significant biological roles, and some of them could be exploited as future biomarkers. In this context, blood is one of the most useful biofluids for screening, but a systematic investigation of the endogenous peptides, especially phosphorylated ones, is still lacking, mainly due to the lack of suitable analytical methods. Thus, in this paper, different phosphopeptide enrichment strategies were pursued, based either on metal oxide affinity chromatography (MOAC, in the form of commercial TiO2 spin columns or magnetic graphitized carbon black-TiO2 composite), or on immobilized metal ion affinity chromatography (IMAC, in the form of Ti4+-IMAC magnetic material or commercial Fe3+-IMAC spin columns). While MOAC strategies proved completely unsuccessful, probably due to interfering phospholipids displacing phosphopeptides, the IMAC materials performed very well. Different sample preparation strategies were tested, comprising direct dilution with the loading buffer, organic solvent precipitation, and lipid removal from the matrix, as well as the addition of phosphatase inhibitors during sample handling for maximized endogenous phosphopeptide enrichment. All data were acquired by a shotgun peptidomics approach, in which peptide samples were separated by reversed-phase nanoHPLC hyphenated with high-resolution tandem mass spectrometry. The devised method allowed the identification of 176 endogenous phosphopeptides in fresh serum added with inhibitors by the direct dilution protocol and the Ti4+-IMAC magnetic material enrichment, but good results could also be obtained from the commercial Fe3+-IMAC spin column adapted to the batch enrichment protocol.

KW - Endogenous serum phosphopeptides

KW - Magnetic solid-phase extraction

KW - NanoHPLC-MS/MS

KW - Phosphopeptide enrichment

KW - Phosphoproteomics

KW - Ti-IMAC

UR - http://www.scopus.com/inward/record.url?scp=85040228507&partnerID=8YFLogxK

U2 - 10.1007/s00216-017-0822-8

DO - 10.1007/s00216-017-0822-8

M3 - Journal article

C2 - 29318361

AN - SCOPUS:85040228507

VL - 410

SP - 1177

EP - 1185

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

SN - 1618-2642

IS - 3

ER -

ID: 231313460