Cytosolic ROS production by NADPH oxidase 2 regulates muscle glucose uptake during exercise

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Standard

Cytosolic ROS production by NADPH oxidase 2 regulates muscle glucose uptake during exercise. / Henríquez-Olguin, Carlos; Knudsen, Jonas Roland; Raun, Steffen Henning; Li, Zhencheng; Dalbram, Emilie; Treebak, Jonas Thue; Sylow, Lykke; Holmdahl, Rikard; Richter, Erik A.; Jaimovich, Enrique; Jensen, Thomas Elbenhardt.

I: Nature Communications, Bind 10, 4623, 2019.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Henríquez-Olguin, C, Knudsen, JR, Raun, SH, Li, Z, Dalbram, E, Treebak, JT, Sylow, L, Holmdahl, R, Richter, EA, Jaimovich, E & Jensen, TE 2019, 'Cytosolic ROS production by NADPH oxidase 2 regulates muscle glucose uptake during exercise', Nature Communications, bind 10, 4623. https://doi.org/10.1038/s41467-019-12523-9

APA

Henríquez-Olguin, C., Knudsen, J. R., Raun, S. H., Li, Z., Dalbram, E., Treebak, J. T., ... Jensen, T. E. (2019). Cytosolic ROS production by NADPH oxidase 2 regulates muscle glucose uptake during exercise. Nature Communications, 10, [4623]. https://doi.org/10.1038/s41467-019-12523-9

Vancouver

Henríquez-Olguin C, Knudsen JR, Raun SH, Li Z, Dalbram E, Treebak JT o.a. Cytosolic ROS production by NADPH oxidase 2 regulates muscle glucose uptake during exercise. Nature Communications. 2019;10. 4623. https://doi.org/10.1038/s41467-019-12523-9

Author

Henríquez-Olguin, Carlos ; Knudsen, Jonas Roland ; Raun, Steffen Henning ; Li, Zhencheng ; Dalbram, Emilie ; Treebak, Jonas Thue ; Sylow, Lykke ; Holmdahl, Rikard ; Richter, Erik A. ; Jaimovich, Enrique ; Jensen, Thomas Elbenhardt. / Cytosolic ROS production by NADPH oxidase 2 regulates muscle glucose uptake during exercise. I: Nature Communications. 2019 ; Bind 10.

Bibtex

@article{ff12ad346d2f41a08a0fa0cb7d5f6f23,
title = "Cytosolic ROS production by NADPH oxidase 2 regulates muscle glucose uptake during exercise",
abstract = "Reactive oxygen species (ROS) act as intracellular compartmentalized second messengers, mediating metabolic stress-adaptation. In skeletal muscle fibers, ROS have been suggested to stimulate glucose transporter 4 (GLUT4)-dependent glucose transport during artificially evoked contraction ex vivo, but whether myocellular ROS production is stimulated by in vivo exercise to control metabolism is unclear. Here, we combined exercise in humans and mice with fluorescent dyes, genetically-encoded biosensors, and NADPH oxidase 2 (NOX2) loss-of-function models to demonstrate that NOX2 is the main source of cytosolic ROS during moderate-intensity exercise in skeletal muscle. Furthermore, two NOX2 loss-of-function mouse models lacking either p47phox or Rac1 presented striking phenotypic similarities, including greatly reduced exercise-stimulated glucose uptake and GLUT4 translocation. These findings indicate that NOX2 is a major myocellular ROS source, regulating glucose transport capacity during moderate-intensity exercise.",
keywords = "Faculty of Science, Reactive oxygen species (ROS), Skeletal muscle, GLUT4, NADPH oxidase 2 (NOX2), Glucose transport, Exercise",
author = "Carlos Henr{\'i}quez-Olguin and Knudsen, {Jonas Roland} and Raun, {Steffen Henning} and Zhencheng Li and Emilie Dalbram and Treebak, {Jonas Thue} and Lykke Sylow and Rikard Holmdahl and Richter, {Erik A.} and Enrique Jaimovich and Jensen, {Thomas Elbenhardt}",
note = "CURIS 2019 NEXS 336",
year = "2019",
doi = "10.1038/s41467-019-12523-9",
language = "English",
volume = "10",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Cytosolic ROS production by NADPH oxidase 2 regulates muscle glucose uptake during exercise

AU - Henríquez-Olguin, Carlos

AU - Knudsen, Jonas Roland

AU - Raun, Steffen Henning

AU - Li, Zhencheng

AU - Dalbram, Emilie

AU - Treebak, Jonas Thue

AU - Sylow, Lykke

AU - Holmdahl, Rikard

AU - Richter, Erik A.

AU - Jaimovich, Enrique

AU - Jensen, Thomas Elbenhardt

N1 - CURIS 2019 NEXS 336

PY - 2019

Y1 - 2019

N2 - Reactive oxygen species (ROS) act as intracellular compartmentalized second messengers, mediating metabolic stress-adaptation. In skeletal muscle fibers, ROS have been suggested to stimulate glucose transporter 4 (GLUT4)-dependent glucose transport during artificially evoked contraction ex vivo, but whether myocellular ROS production is stimulated by in vivo exercise to control metabolism is unclear. Here, we combined exercise in humans and mice with fluorescent dyes, genetically-encoded biosensors, and NADPH oxidase 2 (NOX2) loss-of-function models to demonstrate that NOX2 is the main source of cytosolic ROS during moderate-intensity exercise in skeletal muscle. Furthermore, two NOX2 loss-of-function mouse models lacking either p47phox or Rac1 presented striking phenotypic similarities, including greatly reduced exercise-stimulated glucose uptake and GLUT4 translocation. These findings indicate that NOX2 is a major myocellular ROS source, regulating glucose transport capacity during moderate-intensity exercise.

AB - Reactive oxygen species (ROS) act as intracellular compartmentalized second messengers, mediating metabolic stress-adaptation. In skeletal muscle fibers, ROS have been suggested to stimulate glucose transporter 4 (GLUT4)-dependent glucose transport during artificially evoked contraction ex vivo, but whether myocellular ROS production is stimulated by in vivo exercise to control metabolism is unclear. Here, we combined exercise in humans and mice with fluorescent dyes, genetically-encoded biosensors, and NADPH oxidase 2 (NOX2) loss-of-function models to demonstrate that NOX2 is the main source of cytosolic ROS during moderate-intensity exercise in skeletal muscle. Furthermore, two NOX2 loss-of-function mouse models lacking either p47phox or Rac1 presented striking phenotypic similarities, including greatly reduced exercise-stimulated glucose uptake and GLUT4 translocation. These findings indicate that NOX2 is a major myocellular ROS source, regulating glucose transport capacity during moderate-intensity exercise.

KW - Faculty of Science

KW - Reactive oxygen species (ROS)

KW - Skeletal muscle

KW - GLUT4

KW - NADPH oxidase 2 (NOX2)

KW - Glucose transport

KW - Exercise

U2 - 10.1038/s41467-019-12523-9

DO - 10.1038/s41467-019-12523-9

M3 - Journal article

C2 - 31604916

VL - 10

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 4623

ER -

ID: 228730249