Characterization of oligomeric and kinetic properties of tomato thymidine kinase 1
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
The gene encoding thymidine kinase 1 from tomato (to TK1) has in combination with azidothymidine (AZT) recently been proposed as a powerful suicide gene for anticancer gene therapy. The to TK1/AZT combination has been demonstrated to have several advantages for the treatment of glioblastomas because AZT can easily penetrate the blood-brain barrier and to TK1 can efficiently phosphorylate AZT and also AZT-monophosphate. In a pursuit to further understand the properties of to TK1, we examined the oligomerization properties of recombinant to TK1 and its effect on enzyme kinetics. Previously, it has been shown that human TK1 is a dimer in the absence of ATP and a tetramer if preincubated with ATP. However, we show here that ATP preincubation did not result in a structural shift from dimer to tetramer in to TK1. For human TK1 pretreated with ATP, the Km value decreased 20-fold, but to TK1's Km value did not show a dependence on the presence or absence of ATP. Furthermore, to TK1 was always found in a highly active form.
Originalsprog | Engelsk |
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Tidsskrift | Nucleosides, Nucleotides and Nucleic Acids |
Vol/bind | 30 |
Udgave nummer | 12 |
Sider (fra-til) | 1223-1226 |
Antal sider | 4 |
ISSN | 1525-7770 |
DOI | |
Status | Udgivet - 2011 |
Eksternt udgivet | Ja |
Bibliografisk note
(Ekstern)
ID: 255884357