Beta2-adrenoceptor agonist salbutamol increases protein turnover rates and alters signalling in skeletal muscle after resistance exercise in young men

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Standard

Beta2-adrenoceptor agonist salbutamol increases protein turnover rates and alters signalling in skeletal muscle after resistance exercise in young men. / Hostrup, Morten; Reitelseder, Søren; Jessen, Søren; Kalsen, Anders; Nyberg, Michael Permin; Egelund, Jon; Kreiberg, Michael; Maag Kristensen, Caroline; Thomassen, Martin; Pilegaard, Henriette; Backer, Vibeke; Jacobson, Glenn A; Holm, Lars; Bangsbo, Jens.

I: Journal of Physiology, Bind 596, Nr. 17, 2018, s. 4121-4139.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Hostrup, M, Reitelseder, S, Jessen, S, Kalsen, A, Nyberg, MP, Egelund, J, Kreiberg, M, Maag Kristensen, C, Thomassen, M, Pilegaard, H, Backer, V, Jacobson, GA, Holm, L & Bangsbo, J 2018, 'Beta2-adrenoceptor agonist salbutamol increases protein turnover rates and alters signalling in skeletal muscle after resistance exercise in young men', Journal of Physiology, bind 596, nr. 17, s. 4121-4139. https://doi.org/10.1113/JP275560

APA

Hostrup, M., Reitelseder, S., Jessen, S., Kalsen, A., Nyberg, M. P., Egelund, J., Kreiberg, M., Maag Kristensen, C., Thomassen, M., Pilegaard, H., Backer, V., Jacobson, G. A., Holm, L., & Bangsbo, J. (2018). Beta2-adrenoceptor agonist salbutamol increases protein turnover rates and alters signalling in skeletal muscle after resistance exercise in young men. Journal of Physiology, 596(17), 4121-4139. https://doi.org/10.1113/JP275560

Vancouver

Hostrup M, Reitelseder S, Jessen S, Kalsen A, Nyberg MP, Egelund J o.a. Beta2-adrenoceptor agonist salbutamol increases protein turnover rates and alters signalling in skeletal muscle after resistance exercise in young men. Journal of Physiology. 2018;596(17):4121-4139. https://doi.org/10.1113/JP275560

Author

Hostrup, Morten ; Reitelseder, Søren ; Jessen, Søren ; Kalsen, Anders ; Nyberg, Michael Permin ; Egelund, Jon ; Kreiberg, Michael ; Maag Kristensen, Caroline ; Thomassen, Martin ; Pilegaard, Henriette ; Backer, Vibeke ; Jacobson, Glenn A ; Holm, Lars ; Bangsbo, Jens. / Beta2-adrenoceptor agonist salbutamol increases protein turnover rates and alters signalling in skeletal muscle after resistance exercise in young men. I: Journal of Physiology. 2018 ; Bind 596, Nr. 17. s. 4121-4139.

Bibtex

@article{794c87fa1ca64e4fa08cebcc95c79b2c,
title = "Beta2-adrenoceptor agonist salbutamol increases protein turnover rates and alters signalling in skeletal muscle after resistance exercise in young men",
abstract = "The effect of beta2-adrenoceptor stimulation on skeletal muscle protein turnover and intracellular signalling is insufficiently explored in humans, particularly in association with exercise. In a randomized, placebo-controlled, cross-over study investigating 12 trained men, the effects of beta2-agonist (6 × 4 mg oral salbutamol) on protein turnover rates, intracellular signalling and mRNA response in skeletal muscle were investigated 0.5-5 h after quadriceps resistance exercise. Each trial was preceded by a 4-day lead-in treatment period. Leg protein turnover rates were assessed by infusion of [13C6]-phenylalanine and sampling of arterial and venous blood, as well as vastus lateralis muscle biopsies 0.5 and 5 h after exercise. Furthermore, myofibrillar fractional synthesis rate, intracellular signalling and mRNA response were measured in muscle biopsies. The mean (95% confidence interval) myofibrillar fractional synthesis rate was higher for salbutamol than placebo [0.079 (95% CI, 0.064 to 0.093) vs. 0.066 (95% CI, 0.056 to 0.075%) × h-1] (P < 0.05). Mean net leg phenylalanine balance 0.5-5 h after exercise was higher for salbutamol than placebo [3.6 (95% CI, 1.0 to 6.2 nmol) × min-1 × 100 gLeg Lean Mass -1] (P < 0.01). Phosphorylation of Akt2, cAMP response element binding protein and PKA substrate 0.5 and 5 h after exercise, as well as phosphorylation of eEF2 5 h after exercise, was higher (P < 0.05) for salbutamol than placebo. Calpain-1, Forkhead box protein O1, myostatin and Smad3 mRNA content was higher (P < 0.01) for salbutamol than placebo 0.5 h after exercise, as well as Forkhead box protein O1 and myostatin mRNA content 5 h after exercise, whereas ActivinRIIB mRNA content was lower (P < 0.01) for salbutamol 5 h after exercise. These observations suggest that beta2-agonist increases protein turnover rates in skeletal muscle after resistance exercise in humans, with concomitant cAMP/PKA and Akt2 signalling, as well as modulation of mRNA response of growth-regulating proteins.",
keywords = "Adrenergic, Adrenoceptor, Albuterol, Beta-agonists, Doping, Hypertrophy, LABA, Metabolism, SABA, Strength, Training",
author = "Morten Hostrup and S{\o}ren Reitelseder and S{\o}ren Jessen and Anders Kalsen and Nyberg, {Michael Permin} and Jon Egelund and Michael Kreiberg and {Maag Kristensen}, Caroline and Martin Thomassen and Henriette Pilegaard and Vibeke Backer and Jacobson, {Glenn A} and Lars Holm and Jens Bangsbo",
note = "CURIS 2018 NEXS 268",
year = "2018",
doi = "10.1113/JP275560",
language = "English",
volume = "596",
pages = "4121--4139",
journal = "The Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "17",

}

RIS

TY - JOUR

T1 - Beta2-adrenoceptor agonist salbutamol increases protein turnover rates and alters signalling in skeletal muscle after resistance exercise in young men

AU - Hostrup, Morten

AU - Reitelseder, Søren

AU - Jessen, Søren

AU - Kalsen, Anders

AU - Nyberg, Michael Permin

AU - Egelund, Jon

AU - Kreiberg, Michael

AU - Maag Kristensen, Caroline

AU - Thomassen, Martin

AU - Pilegaard, Henriette

AU - Backer, Vibeke

AU - Jacobson, Glenn A

AU - Holm, Lars

AU - Bangsbo, Jens

N1 - CURIS 2018 NEXS 268

PY - 2018

Y1 - 2018

N2 - The effect of beta2-adrenoceptor stimulation on skeletal muscle protein turnover and intracellular signalling is insufficiently explored in humans, particularly in association with exercise. In a randomized, placebo-controlled, cross-over study investigating 12 trained men, the effects of beta2-agonist (6 × 4 mg oral salbutamol) on protein turnover rates, intracellular signalling and mRNA response in skeletal muscle were investigated 0.5-5 h after quadriceps resistance exercise. Each trial was preceded by a 4-day lead-in treatment period. Leg protein turnover rates were assessed by infusion of [13C6]-phenylalanine and sampling of arterial and venous blood, as well as vastus lateralis muscle biopsies 0.5 and 5 h after exercise. Furthermore, myofibrillar fractional synthesis rate, intracellular signalling and mRNA response were measured in muscle biopsies. The mean (95% confidence interval) myofibrillar fractional synthesis rate was higher for salbutamol than placebo [0.079 (95% CI, 0.064 to 0.093) vs. 0.066 (95% CI, 0.056 to 0.075%) × h-1] (P < 0.05). Mean net leg phenylalanine balance 0.5-5 h after exercise was higher for salbutamol than placebo [3.6 (95% CI, 1.0 to 6.2 nmol) × min-1 × 100 gLeg Lean Mass -1] (P < 0.01). Phosphorylation of Akt2, cAMP response element binding protein and PKA substrate 0.5 and 5 h after exercise, as well as phosphorylation of eEF2 5 h after exercise, was higher (P < 0.05) for salbutamol than placebo. Calpain-1, Forkhead box protein O1, myostatin and Smad3 mRNA content was higher (P < 0.01) for salbutamol than placebo 0.5 h after exercise, as well as Forkhead box protein O1 and myostatin mRNA content 5 h after exercise, whereas ActivinRIIB mRNA content was lower (P < 0.01) for salbutamol 5 h after exercise. These observations suggest that beta2-agonist increases protein turnover rates in skeletal muscle after resistance exercise in humans, with concomitant cAMP/PKA and Akt2 signalling, as well as modulation of mRNA response of growth-regulating proteins.

AB - The effect of beta2-adrenoceptor stimulation on skeletal muscle protein turnover and intracellular signalling is insufficiently explored in humans, particularly in association with exercise. In a randomized, placebo-controlled, cross-over study investigating 12 trained men, the effects of beta2-agonist (6 × 4 mg oral salbutamol) on protein turnover rates, intracellular signalling and mRNA response in skeletal muscle were investigated 0.5-5 h after quadriceps resistance exercise. Each trial was preceded by a 4-day lead-in treatment period. Leg protein turnover rates were assessed by infusion of [13C6]-phenylalanine and sampling of arterial and venous blood, as well as vastus lateralis muscle biopsies 0.5 and 5 h after exercise. Furthermore, myofibrillar fractional synthesis rate, intracellular signalling and mRNA response were measured in muscle biopsies. The mean (95% confidence interval) myofibrillar fractional synthesis rate was higher for salbutamol than placebo [0.079 (95% CI, 0.064 to 0.093) vs. 0.066 (95% CI, 0.056 to 0.075%) × h-1] (P < 0.05). Mean net leg phenylalanine balance 0.5-5 h after exercise was higher for salbutamol than placebo [3.6 (95% CI, 1.0 to 6.2 nmol) × min-1 × 100 gLeg Lean Mass -1] (P < 0.01). Phosphorylation of Akt2, cAMP response element binding protein and PKA substrate 0.5 and 5 h after exercise, as well as phosphorylation of eEF2 5 h after exercise, was higher (P < 0.05) for salbutamol than placebo. Calpain-1, Forkhead box protein O1, myostatin and Smad3 mRNA content was higher (P < 0.01) for salbutamol than placebo 0.5 h after exercise, as well as Forkhead box protein O1 and myostatin mRNA content 5 h after exercise, whereas ActivinRIIB mRNA content was lower (P < 0.01) for salbutamol 5 h after exercise. These observations suggest that beta2-agonist increases protein turnover rates in skeletal muscle after resistance exercise in humans, with concomitant cAMP/PKA and Akt2 signalling, as well as modulation of mRNA response of growth-regulating proteins.

KW - Adrenergic

KW - Adrenoceptor

KW - Albuterol

KW - Beta-agonists

KW - Doping

KW - Hypertrophy

KW - LABA

KW - Metabolism

KW - SABA

KW - Strength

KW - Training

U2 - 10.1113/JP275560

DO - 10.1113/JP275560

M3 - Journal article

C2 - 29968301

AN - SCOPUS:85051025375

VL - 596

SP - 4121

EP - 4139

JO - The Journal of Physiology

JF - The Journal of Physiology

SN - 0022-3751

IS - 17

ER -

ID: 200968645