TY - JOUR

T1 - A rapid magnetic solid phase extraction method followed by liquid chromatography-tandem mass spectrometry analysis for the determination of mycotoxins in cereals

AU - La Barbera, Giorgia

AU - Capriotti, Anna Laura

AU - Cavaliere, Chiara

AU - Foglia, Patrizia

AU - Montone, Carmela Maria

AU - Zenezini Chiozzi, Riccardo

AU - Laganà, Aldo

N1 - (Ekstern)

PY - 2017

Y1 - 2017

N2 - Mycotoxins can contaminate various food commodities, including cereals. Moreover, mycotoxins of different classes can co-contaminate food, increasing human health risk. Several analytical methods have been published in the literature dealing with mycotoxins determination in cereals. Nevertheless, in the present work, the aim was to propose an easy and effective system for the extraction of six of the main mycotoxins from corn meal and durum wheat flour, i.e., the main four aflatoxins, ochratoxin A, and the mycoestrogen zearalenone. The developed method exploited magnetic solid phase extraction (SPE), a technique that is attracting an increasing interest as an alternative to classical SPE. Therefore, the use of magnetic graphitized carbon black as a suitable extracting material was tested. The same magnetic material proved to be effective in the extraction of mycoestrogens from milk, but has never been applied to complex matrices as cereals. Ultra high-performance liquid chromatography tandem mass spectrometry was used for detection. Recoveries were >60% in both cereals, even if the matrix effects were not negligible. The limits of quantification of the method results were comparable to those obtained by other two magnetic SPE-based methods applied to cereals, which were limited to one or two mycotoxins, whereas in this work the investigated mycotoxins belonged to three different chemical classes.

AB - Mycotoxins can contaminate various food commodities, including cereals. Moreover, mycotoxins of different classes can co-contaminate food, increasing human health risk. Several analytical methods have been published in the literature dealing with mycotoxins determination in cereals. Nevertheless, in the present work, the aim was to propose an easy and effective system for the extraction of six of the main mycotoxins from corn meal and durum wheat flour, i.e., the main four aflatoxins, ochratoxin A, and the mycoestrogen zearalenone. The developed method exploited magnetic solid phase extraction (SPE), a technique that is attracting an increasing interest as an alternative to classical SPE. Therefore, the use of magnetic graphitized carbon black as a suitable extracting material was tested. The same magnetic material proved to be effective in the extraction of mycoestrogens from milk, but has never been applied to complex matrices as cereals. Ultra high-performance liquid chromatography tandem mass spectrometry was used for detection. Recoveries were >60% in both cereals, even if the matrix effects were not negligible. The limits of quantification of the method results were comparable to those obtained by other two magnetic SPE-based methods applied to cereals, which were limited to one or two mycotoxins, whereas in this work the investigated mycotoxins belonged to three different chemical classes.

KW - Aflatoxins

KW - Cereals

KW - Graphitized carbon black

KW - Liquid chromatography-tandem mass spectrometry

KW - Magnetic solid phase extraction

KW - Maize

KW - Mycotoxins

KW - Ochratoxin A

KW - Wheat

KW - Zearalenone

UR - http://www.scopus.com/inward/record.url?scp=85018301862&partnerID=8YFLogxK

U2 - 10.3390/toxins9040147

DO - 10.3390/toxins9040147

M3 - Journal article

C2 - 28430148

AN - SCOPUS:85018301862

VL - 9

JO - Toxins

JF - Toxins

SN - 2072-6651

IS - 4

M1 - 147

ER -